Comprehensive test kit measures three fundamental elements required for coral health. Red Sea Reef Foundation Pro Multi Test Kit takes a fresh approach to greater testing convenience and accuracy. Included Red Sea Calcium Pro, KH/Alkalinity Pro and Magnesium Pro Tests are packaged together at an outstanding value. New and experienced reef hobbyists alike can enjoy this precision test kit that simplifies the titration (color change) testing method with an easy-to-use, single hand titrator.

Red Sea Reef Foundation Pro Multi Test Kit contains three tests selected specifically to accurately measure chemical elements associated with proper marine water chemistry AND coral growth. Calcium (Ca), Magnesium (Mg) and Carbonates (CO3) has a major effect on pH stability and alkalinity. These elements also need to be available in balanced proportions for healthy growth of coral skeletons.

Included quick guide walks you through the testing process with simple, easy-to-understand illustrations. Reverse features a chart that displays test results. Routine testing with Red Sea Reef Foundation Pro Multi Test Kit is crucial to the health and success of any coral reef aquarium housing SPS, LPS and Soft Corals, especially those on a supplementation regimen.

Red Sea Reef Foundation Pro Multi Test Kit Contents:

• Titrator (3 Syringe 1 ml)
• Instruction Manual
• 3 Color Cards
• Syringe 10ml
• Calcium Reagent A - 25ml
• Calcium Reagent B - 12g
• Calcium Reagent C - 60ml
• Alkalinity Reagent - 60ml
• Magnesium Reagent A - 15ml
• Magnesium Reagent B - 15ml
• Magnesium Reagent C - 60ml

Directions for Calcium Pro Test Kit

1. Using the large syringe provided, place exactly 5 ml of the water to be tested into the glass vial.
2. Add 5 drops of Calcium Pro Reagent A and mix gently with the water sample.
3. Add a leveled measuring spoon of Calcium Pro Reagent B, close the vial with the cap and shake for 20 seconds. The test sample will now have the titration start color as shown on the instruction card.
4. Screw the glass vial onto the bottom of the Titrator.
5. Ensure that the dispensing tip is on the 1 ml titration syringe and fill with 1ml of Calcium Pro Titrant (C). [See note for filling titration syringe.]
6. Insert the syringe into the center of the Titrator so that the volumetric scale on the side of the syringe is visible. The graduations of the volumetric scale are 0.01 ml, equivalent to 5 ppm of calcium.
7. Add the first 0.6 ml of titrant (equivalent to 300ppm of calcium) by pressing the plunger of the syringe while gently swirling the Titrator. Continue adding the titrant gradually, swirling the Titrator after each addition and paying attention to the color in the vial. As the color in the vial approaches the end color as shown on the instruction card, add one drop at a time, swirling after each drop, until the end color is achieved.
   • Note: If you have added 1 ml of Calcium Titrant C, without reaching the end color, the calcium concentration of your water sample is more than 500 ppm. Refill the syringe and continue to add titrant until you reach the end color.
8. Note the amount of titrant used (according to the initial and final position of the plunger not the liquid surface) and use the table to calculate the level of calcium in the water sample.
9. Return any unused quantity of titrant to the Calcium Pro Titrant C bottle.

Directions for KH Alkalinity Pro Test Kit

1. Using the large syringe provided, place exactly 10 ml of the water to be tested into the glass vial.
2. Screw the glass vial onto the bottom of the Titrator.
3. Ensure that the dispensing tip is on the 1 ml titration syringe and fill with 1ml of Alkalinity Pro Titrant. (See note for filling titration syringe.)
4. Insert the syringe into the center of the Titrator so that the volumetric scale on the side of the syringe is visible. The graduations of the volumetric scale are 0.01 ml, equivalent to an alkalinity of 0 .05 meq/L (0.14 dKH).
5. Add the first 0.4 ml of titrant (equivalent to an alkalinity of 2.0 meq/L - 5.6 dKH) by pressing the plunger of the syringe while gently swirling the Titrator. The test sample will obtain the titration start color as shown on the instruction card after the first few drops of titrant are added. Continue adding the titrant gradually, swirling the Titrator after each addition and paying attention to the color in the vial. As the color in the vial approaches the end color as shown on the instruction card, add one drop at a time, swirling after each drop, until the end color is achieved. Note: If you have added 1 ml of alkalinity titrant, without reaching the end color, the alkalinity of your water sample is greater than 5 meq/L (14 dKH). Refill the syringe and continue to add titrant until you reach the end color.
6. Note the amount of titrant used (according to the initial and final position of the plunger not the liquid surface) and use the table on the instruction card to calculate the level of alkalinity in the water sample.
7. Dispose of any unused quantity of titrant.

Directions for Magnesium Pro Test Kit

1. Using the large syringe provided, place exactly 2 ml of the water to be tested into the glass vial.
2. Add 5 drops of Mg Pro Reagent A, shaking the vial for 15 seconds after each drop.
3. Add 5 drops of Mg Pro Reagent B and mix the reagent gently with the water sample.
4. Screw the glass vial onto the bottom of the Titrator and wait for approx 60 seconds for the test sample to obtain the titration start color as shown on the instruction card. A precipitate will form on the bottom of the vial.
5. Ensure that the dispensing tip is on the 1 ml titration syringe and fill with 1ml of Magnesium Pro Titrant (C). [See note for filling titration syringe.]
6. Insert the syringe into the center of the Titrator so that the volumetric scale on the side of the syringe is visible. The graduations of the volumetric scale are 0.01 ml, equivalent to 20 ppm of magnesium.
7. Add the first 0.4 ml of titrant (equivalent to 800ppm of magnesium) by pressing the plunger of the syringe while gently swirling the Titrator. Continue adding the titrant gradually, swirling the Titrator after each addition and paying attention to the color in the vial. As the color in the vial approaches the end color as shown on the instruction card, add one drop at a time, swirling after each drop, until the blue end color is first achieved. Note: Shortly after the blue end color is achieved, the liquid in the vial will turn from blue to violet. Do not continue adding titrant. If you have used more than 0.8ml of titrant, you may have missed the end point and should redo the test.
8. Note the amount of titrant used (according to the initial and final position of the plunger not the liquid surface) and use the table on the instruction card to calculate the level of magnesium in the water sample.
9. Return any unused quantity of titrant to the Magnesium Pro Titrant C bottle.

General Information

The Foundation Elements (Ca, KH & Mg)
A successful coral reef aquarium is dependent upon maintaining the appropriate water parameters that in turn provide the stable environment required by the corals. Although all the elements found in natural seawater have an important role in providing the optimal water parameters, a few of them have a more significant role in the overall stability. These elements are the foundation of the reef environment and they include the three major elements, Calcium (Ca), Magnesium (Mg) and Bi-Carbonates (HCO3). These three elements have a major effect on the water chemistry (pH stability, Alkalinity and seawater ionic strength) and on many of the coral's biological processes (skeleton formation, ion exchange and photosynthesis).

Red Sea Reef Care Program
General Instructions for Testing and Supplementing

• Before carrying out any water testing, always check the salinity and make adjustments as necessary. If you have made adjustments to the water, wait 10 minutes for the water parameters to stabilize. (E.g. 1 ppt increase in salinity due to evaporation of freshwater will result in approximate increases of 13ppm Ca and 40ppm Mg).
• Test only with high resolution test kits such as Red Sea's Foundation Program (Calcium, Alkalinity & Magnesium) Pro Titration Kits.
• All of the Red Sea Reef Care Program supplements have dosing charts (on back of product) based on treating 100 liters / 25 gallons of water. Estimate your total volume of water (aquarium & sump less volume of live rocks etc) to calculate the correct dosage for your system.
• Supplements should be added to the sump. If you do not have a sump, add the supplements slowly to an area with high water flow to prevent direct contact with fish and corals.
• To prevent stress to the corals, the maximum daily increases of each of the elements are as follows: Calcium 20ppm; Alkalinity 0.5 meq/l (1.4 dkh); Magnesium 10ppm. Larger adjustments should be spread over a few days according to the daily maximum.
• In order to prevent the effects of unbalanced levels of the foundation elements, supplements should be added in the following order allowing 10 minutes between each:
  • 1st - Magnesium
  • 2nd - Alkalinity
  • 3rd - Calcium

Important Notes for Using the Reef Foundation Titration Test Kits

• Before testing, clean the glass vial and the large syringe by rinsing with the water to be tested.
• After testing, rinse all syringes and vials with RO or distilled water before storing. If vials are left unwashed, a residue can form that will affect the results of future tests. Use a slightly acidic solution such as vinegar to remove the residue.
• Close all reagents tightly immediately after use.
• The test reagents are stable up to the date stated on the pack when stored closed between 15 - 25°C (59 - 77°F).
• Store the reagents in the plastic box to prevent damage from prolonged exposure to light.

Note for Filling the 1ml Titration Syringe
Raise the plunger of the syringe until the bottom ring of the plunger is at the 1.0 ml mark. The surface of the liquid will be approximately 0.1 - 0.15 ml below the plunger.

Do not try to remove the air trapped between the liquid and the plunger. This small volume of air corresponds to the liquid held inside the plastic tip.